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Cell growth curve Cell proliferation was determined by the IncuCyte ZOOM Live-Cell Imaging system (Essen BioScience, MI, USA)

Second, by modifying specific functional molecules on the surface of the coating, the adhesion and colonization ability can increase the survivability of probiotic strains, thereby supporting their function in the gut, such as gastric acid, bile salts, and the viability of probiotics on the intestinal mucosa can be enhanced, and their residence time in the intestinal tract can be prolonged, thus exerting a more lasting probiotic effect [142,143,144]

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In clinical practice, the treatment based on regulating intestinal microbiota has been applied to inflammatory bowel disease [16], irritable bowel syndrome [17], and liver cirrhosis [18], which has become a hot research topic in the world