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To replace the gene, 5 and 3 flanking regions of the pbggcs gene were cloned up- and downstream of the selection cassette of plasmid pL0001 (Malaria Research and Reference Reagent Resource Center: MR-4) that contains the Toxoplasma gondii dihydrofolate reductasethymidylate synthase ( tgdhfr / ts ) selectable marker cassette

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Krystal AD, Durrence HH, Scharf M, et al