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Given the anatomical reality that the brainstem comprises many densely-packed but functionally-heterogeneous nuclei, any small errors in spatial registration caused by failure to use brainstem-specific registration are highly likely to lead to decreased sensitivity in signal and type 2 errors ( Furthermore, the brainstem is especially prone to physiologically-driven movement artifact given that it pulses with every heartbeat [e.g., ( A large majority of neuroimaging studies in ME/CFS have not used brainstem-specific spatial co-registration, normalization, or physiologically-derived movement artifact regression techniques

MicroRNAs as potential diagnostic biomarkers for bipolar disorder

Methods: Sandwich ELISAs measuring the matricellular fibrosis markers thrombospondin-2 (TSP2) and thrombospondin-4 (TSP4) and the metabolism/inflammation/fibrosis-related markers insulin-like growth factor binding protein-7 (IGFBP7), CD163 and a disintegrin and metalloprotease thrombospondin like-9 (ADAMTS9) were established in-house using combinations of recombinantly expressed proteins, commercial and self-produced monoclonal and polyclonal antibodies