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CLSM analysis of hypocotyl sections of plants exposed to abiotic stress (mechanical wounding and high temperatures) showed a general accumulation of GSNO in all hypocotyl cells, with a concomitant reduction in GSNOR activity, thus mediating nitrosative stress (Chaki et al., Plasmopara halstedii

Imaging was performed using a Philips Mosaic tomograph (Cleveland, OH, USA), with anatomical images acquired in a U-SPECT6/CT scanner (MILabs)

This is true for both proteins in purification buffer as well as in the coupling buffer used for the pull-down experiments