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The tissues were then centrifuged at 5000 rpm for 5 minutes to collect the supernatant in Eppendorf tubes, and stored at -20 C for biochemical tests

doi: 10.1111/acel.2015.14.issue-5 Summary Keywords oxidative stress, sperm dysfunction, reactive oxygen species, DNA fragmentation and male infertility, male infertility Citation Wang Y, Fu X and Li H (2025) Mechanisms of oxidative stress-induced sperm dysfunction

Additionally, dysfunctional Nrf2 cannot effectively regulate immune responses, leading to the excessive release of inflammatory factors, which worsens joint inflammation and damage