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Xiao Y-F, Jie M-M, Li B-S, Hu C-J, Xie R, Tang B, et al

Application of MS to large polar organic molecules, such as proteins and other biomolecules, was originally limited by the fact that the ionization techniques available at the time were too violent (Clarke, 2019)

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Monitoring response to treatment involves: Clinical assessment of symptom improvement, including mouth ulcer frequency FBC to confirm resolution of anaemia and reticulocyte response Ongoing monitoring of neurological symptoms if present Routine repeat measurement of serum B12 levels shortly after starting injections is not generally recommended as results may be misleadingly high