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Furthermore, randomised clinical trials and observational studies have indicated that the perimenopausal phase is particularly associated with memory decline and an increased risk of mild cognitive impairment and dementia 31,32,33,34,35,36

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The pretreated cells were digested with EDTA-free trypsin and washed twice with PBS, the remaining operations were carried out according to the instructions of the S0053, S0033S and S0131S (Beyotime Biotechnology, Shanghai, China) kits

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