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Cells were dehydrated in a series of alcoholic solutions from 70 to 100%, followed by two immersions in propylene oxide for 20 min each, propylene oxide and araldite infiltration for 12 h, at room temperature on a shaking platform

10.1152/physrev.00010.2018 28 BhattiJ

By blocking ME2 to lower NADPH levels and adding BSO (GSH synthesis inhibitor) to enhance the ROS effect in concert, the oxidative defense barrier of AML cells was successfully broken (113, 157)