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However, it should be noted that among the amino acids present in breast milk, circadian fluctuations are observed particularly in tryptophan, with levels rising at night, peaking in the early morning hours, and reaching their lowest levels in the afternoon [7, 23]

This process is mediated by G-protein-signaling, activating phospholipase C and increasing cytosolic Ca 2+ concentrations

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[DOI] [PubMed] [Google Scholar] 95.Wu D., Wang Y., Yang G., Zhang S., Liu Y., Zhou S., Guo H., Liang S., Cui Y., Zhang B., et al